Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increase de Novo Purine Biosynthesis
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منابع مشابه
Quantitative analysis of purine nucleotides indicates that purinosomes increase de novo purine biosynthesis.
Enzymes in the de novo purine biosynthesis pathway are recruited to form a dynamic metabolic complex referred to as the purinosome. Previous studies have demonstrated that purinosome assembly responds to purine levels in culture medium. Purine-depleted medium or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole (DMAT) treatment stimulates the purinosome assembly in HeLa cells. Here, several m...
متن کاملQuantitative Analysis of Purine Nucleotides Indicates Purinosomes Increase de Novo Purine Biosynthesis
Enzymes in the de novo purine biosynthesis pathway are recruited to form a dynamic metabolic complex referred to as the purinosome. Previous studies have demonstrated that purinosome assembly responds to purine levels in culture medium. Purine-depleted medium or 2-dimethylamino4,5,6,7-tetrabromo-1H-benzimidazole (DMAT) treatment stimulates the purinosome assembly in HeLa cells. Here, several me...
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The carbocyclic analogues of phosphoribosylamine, glycinamide ribonucleotide, and formylglycinamide ribonucleotide have been prepared as the racemates. Carbocyclic phosphoribosylamine was utilized as a substrate by the monofunctional glycinamide ribonucleotide synthetase from Escherichia coli as well as the glycinamide ribonucleotide synthetase activity of the eucaryotic trifunctional enzyme of...
متن کاملThe biosynthesis of purine nucleotides de novo in the rabbit reticulocyte in vitro.
The mature rabbit erythrocyte has been shown to be incapable of synthesizing de novo the purine portions of adenosine triphosphate and of guanosine triphosphate, in vitro (2). It is able, however, to carry out the final steps of this pathway from sodium formate and 5-amino-1-ribosyl-4-imidazolecarboxamide, a finclmg which suggests that a portion of the over-all enzymatic sequence is lost during...
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The capacity of intact cells of roots excised from summer squash plants (Cucurbita pepo L. cv Early Prolific Straightneck) to synthesize purine nucleotides de novo was investigated. Evidence that purine nucleotides are synthesized de novo included: (a) demonstration of the incorporation of [1-(14)C]glycine, [2-(14)C]glycine, NaH(14)CO(3), and H(14)COONa into total adenine nucleotides; (b) obser...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2015
ISSN: 0021-9258
DOI: 10.1074/jbc.m114.628701